Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleoprotein-based nanoscale assembly

S S Smith1, L Niu, D J Baker

  • 1Department of Cell and Tumor Biology, City of Hope National Medical Center, Duarte, CA 91010, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 18, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of risk factors for prolonged postoperative ileus after segmental jejunectomy].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2026
Same author

Left and right sides are needed: seed development improves when both stigma lobes of Habenaria limprichtii (Orchidaceae) are pollinated.

Plant biology (Stuttgart, Germany)·2025
Same author

Intestinal microbiota profiles of captive-bred cynomolgus macaques reveal influence of biogeography and age.

Animal microbiome·2025
Same author

Effect of Electrical Stimulation of Lingual Nerve on Xerostomia: A Randomized Controlled Trial.

JDR clinical and translational research·2024
Same author

Apoptotic Vesicles: Therapeutic Mechanisms and Critical Issues.

Journal of dental research·2024
Same author

[Euonymus alatus delays progression of diabetic kidney disease in mice by regulating EGFR tyrosine kinase inhibitor resistance signaling pathway].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2024
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

This study introduces a novel system for building macromolecular assemblies using DNA methyltransferases. This DNA-based addressing system precisely positions components on DNA, enabling the creation of complex structures.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Macromolecular assemblies are crucial in biological systems.
  • Precise control over component placement is essential for constructing complex molecular architectures.
  • Existing methods for assembling macromolecules have limitations in specificity and order.

Purpose of the Study:

  • To develop a novel system for addressing and assembling macromolecules on DNA.
  • To utilize the biospecificity of DNA methyltransferases for targeted component placement.
  • To demonstrate the system's potential in creating ordered macromolecular structures.

Main Methods:

  • Employed DNA (cytosine-5) methyltransferases (M-HhaI and M x MspI) for targeted DNA modification.
  • Introduced 5-fluorocytosine into synthetic oligodeoxynucleotides to create specific recognition sites.

Related Experiment Videos

  • Created chimeric enzymes by linking a dodecapeptide antigen to M-HhaI to test specificity.
  • Utilized enzyme kinetic footprints and DNA helix screw axis for spatial guidance.

    • Main Results:

    • Demonstrated that modified DNA sequences serve as specific addresses for methyltransferases.
    • Showcased successful targeted delivery of a linked peptide to a DNA recognition site using a chimeric enzyme.
    • Confirmed that methyltransferase specificity is retained in chimeric constructs.
    • Established that component order and spacing on DNA can be controlled.

    Conclusions:

    • The developed DNA-based addressing system enables precise, ordered construction of macromolecular assemblies.
    • This system holds significant potential for creating protein arrays, modeling biological complexes like chromatin, and building novel molecular devices.