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Nucleotide sequence of the vmhA gene encoding hemolysin from Vibrio mimicus
1RCOID and Department of Biotechnology and Bioengineering, Pusan, South Korea.
Biochimica Et Biophysica Acta
|April 12, 1997
Summary
Researchers cloned and sequenced the Vibrio mimicus hemolysin gene (vmhA). This gene codes for a protein highly similar to Vibrio cholerae hemolysin, offering insights into bacterial virulence factors.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Vibrio mimicus is a marine bacterium.
- Hemolysins are virulence factors produced by various bacteria.
- Understanding hemolysin structure is crucial for studying bacterial pathogenesis.
Purpose of the Study:
- To clone and sequence the hemolysin gene (vmhA) from Vibrio mimicus.
- To characterize the structural and predicted protein properties of vmhA.
- To compare the vmhA sequence with known hemolysins.
Main Methods:
- Gene cloning techniques.
- DNA sequencing of the vmhA gene.
- Bioinformatic analysis of nucleotide and amino acid sequences.
Main Results:
- The vmhA gene consists of 2232 nucleotides.
- It encodes a protein of 744 amino acids with a molecular mass of 83,059 Da.
- The deduced amino acid sequence exhibits 81.6% identity to Vibrio cholerae El Tor hemolysin.
Conclusions:
- The vmhA gene is successfully cloned and sequenced.
- The Vibrio mimicus hemolysin shares significant sequence homology with Vibrio cholerae hemolysin.
- This finding contributes to the understanding of hemolysin evolution and function in Vibrio species.