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Defined epitopes: in vivo and in vitro studies using recombinant allergens
M D Chapman1, A M Smith, L D Vailes
1Asthma and Allergic Diseases Center, Department of Medicine, University of Virginia, Charlottesville 22908, USA. mdc8m@virginia.edu
International Archives of Allergy and Immunology
|May 1, 1997
Summary
Recombinant cockroach and mite allergens are biologically active and useful for diagnosing and treating allergies. These findings suggest improved management of allergic diseases through recombinant allergen cocktails.
Area of Science:
- Allergen immunotherapy
- Molecular biology
- Immunology
Background:
- Allergic diseases pose a significant health burden.
- Current diagnostic and therapeutic strategies for allergies can be improved.
- Recombinant allergens offer a potential avenue for enhanced allergy management.
Purpose of the Study:
- To assess the biological activity of recombinant cockroach and mite allergens.
- To evaluate their utility in diagnosing and treating allergic conditions.
Main Methods:
- Production of recombinant cockroach allergens (Bla g 2, Bla g 4, Bla g 5) and mite group 5 allergens using bacterial expression vectors.
- Immediate skin tests and serum IgE antibody tests were performed.
Main Results:
- Recombinant cockroach allergens demonstrated high skin test reactivity in allergic patients.
- Reactivity correlated with serum IgE antibody levels.
- Sensitization to Blomia tropicalis allergen (Blo t 5) varied geographically, with higher prevalence in Brazil and Singapore (70%) compared to the US and UK (<20%).
Conclusions:
- Recombinant allergens maintain biological activity.
- Cocktails of recombinant allergens show promise for diagnostic and therapeutic applications.
- Phased introduction of recombinant allergens could enhance allergic disease management.