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Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
Published on: July 4, 2018
An in vitro model to assess mucosal immune function and bacterial translocation
L N Diebel1, D M Liberati, S A Dulchavsky
1Wayne State University, Department of Surgery and Pathology, Detroit, Michigan 48201, USA.
The Journal of Surgical Research
|April 1, 1997
Summary
Secretory immunoglobulin A (sIgA) significantly reduces bacterial translocation across intestinal epithelial cells. This study presents a novel in vitro model to investigate sIgA
Area of Science:
- Immunology
- Cell Biology
- Microbiology
Background:
- Secretory immunoglobulin A (sIgA) plays a crucial role in intestinal barrier function.
- Studying the in vivo role of sIgA in mucosal defense is challenging due to the complex intestinal microenvironment.
Purpose of the Study:
- To investigate the impact of secretory immunoglobulin A (sIgA) on bacterial translocation (BT) using an in vitro model.
- To establish a reliable cell culture system for studying sIgA's role in intestinal barrier integrity.
Main Methods:
- Utilized polarized Madin-Darby canine kidney (MDCK) epithelial cells in a two-chamber system.
- Expressed the polymeric Ig receptor cDNA in MDCK cells.
- Assessed bacterial translocation by measuring passage across cell monolayers after apical inoculation with bacteria in the presence or absence of sIgA.
Main Results:
- Bacterial passage across MDCK monolayers demonstrated a time and inoculum concentration-dependent gradient.
- The addition of sIgA resulted in a significant reduction in bacterial translocation.
- The in vitro model effectively demonstrated the protective effect of sIgA.
Conclusions:
- Secretory immunoglobulin A (sIgA) effectively inhibits bacterial translocation across epithelial monolayers.
- This in vitro model provides a valuable tool for further research into sIgA's function in intestinal barrier defense.
