Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Supramolecular architectures for the functionalization of solid surfaces

W Knoll1, M Liley, D Piscevic

  • 1Max-Planck-Institut für Polymerforschung, Mainz, Germany.

Advances in Biophysics
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Olfactory dysfunction in SARS-CoV-2 infection: Focus on odorant specificity and chronic persistence.

American journal of otolaryngology·2021
Same author

A surface plasmon field-enhanced fluorescence reversible split aptamer biosensor.

The Analyst·2017
Same author

Influence of myelin proteins on the structure and dynamics of a model membrane with emphasis on the low temperature regime.

The Journal of chemical physics·2014
Same author

Design and test of a simple high-temperature laser microrefractometer.

Applied optics·2010
Same author

Unusual splitting behavior of the dispersion of surface polaritons in gratings of different symmetry, amplitude, and profile.

Applied optics·2010
Same author

The quantification of single cell adhesion on functionalized surfaces for cell sheet engineering.

Biomaterials·2010

Surface plasmon optics enable real-time analysis of bio-interfaces. Researchers optimized self-assembled monolayers (SAMs) for enhanced protein binding using specific bio-recognition reactions and UV-photolithography.

Area of Science:

  • Surface science
  • Biophysics
  • Nanotechnology

Background:

  • Surface plasmon optical techniques offer sensitive, real-time characterization of biointerfaces.
  • Supramolecular biofunctional architectures are crucial for various biosensing applications.

Purpose of the Study:

  • To characterize the build-up of functional bio-interfaces via self-assembly.
  • To develop criteria for optimizing self-assembled monolayer (SAM) architectures for protein binding.
  • To demonstrate selective protein binding on patterned SAMs.

Main Methods:

  • Surface plasmon optics for interface characterization.
  • Self-assembly of long-chain thiolates on gold surfaces.
  • Scanning probe microscopy (SPM) for imaging.

Related Experiment Videos

  • UV-photolithographic patterning for lateral functionalization.

    • Main Results:

    • Demonstrated the self-assembly process of thiolates forming a functional bio-interface.
    • Established criteria for tailoring SAMs to maximize protein binding.
    • Successfully imaged selective streptavidin binding to patterned SAMs.

    Conclusions:

    • Surface plasmon techniques are effective for on-line characterization of bio-interfaces.
    • Tailored SAMs can significantly enhance specific protein capture.
    • Combined techniques allow for precise control and imaging of bio-recognition events.