Related Experiment Videos
Effect of tissue and plasma factors on kidney proliferation
A M Inda1, A L García, A L Errecalde
1Instituto de Embriología, Biología e Histología Facultad de Ciencias Médicas, UNLP, La Plata, Argentina.
Abstract:
Liver extract, plasma from intact mice, ES2 tumour extract and plasma from tumour bearing mice has an inhibiting effect on the mitotic activity of hepatocytes and duodenal enterocytes. In the present experiments, the effect of these treatments on the mitotic activity of renal tubular cells was studied. C3HS 28 day-old male mice, standardized for periodicity analysis were used. The determination of normal mitotic circadian curve of the renocytes was done. A second batch of mice were injected with 0.01 ml/gr of either liver extract, plasma from intact mice, ES2 tumour extract or plasma from tumour bearing mice, at 16:00 hours and controlled at 08:00, 12:00 and 16:00 hs during 2 consecutive days post treatment. Colchicine (2 micrograms/gr) was injected 4 hours before killing. Kidneys were processed for histology and mitotic index determinations. Results were expressed as colchicine metaphases per 1000 nuclei, and showed that mitotic activity values of treated animals were significantly lower than those of controls. In conclusion, mitotic activity inhibition of renocytes may be due to some non specific plasmatic and/or tissue factors.
Insights
Liver extract and tumor extracts inhibit cell division in mice. These substances, including plasma from tumor-bearing mice, significantly reduce mitotic activity in renal tubular cells, suggesting non-specific inhibitory factors.
Area of Science:
- Cell Biology
- Oncology
- Physiology
Background:
- Liver extract, plasma from intact mice, ES2 tumor extract, and plasma from tumor-bearing mice are known to inhibit mitotic activity in hepatocytes and duodenal enterocytes.
- The effect of these substances on renal tubular cells (renocytes) has not been previously investigated.
Purpose of the Study:
- To investigate the effect of liver extract, plasma from intact mice, ES2 tumor extract, and plasma from tumor-bearing mice on the mitotic activity of renal tubular cells.
- To establish the normal mitotic circadian curve of renocytes in C3HS mice.
Main Methods:
- Determination of the normal mitotic circadian curve of renocytes in 28-day-old male C3HS mice.
- Injection of liver extract, plasma from intact mice, ES2 tumor extract, or plasma from tumor-bearing mice into mice at 16:00 hours.
- Administration of colchicine 4 hours prior to tissue collection for histological processing and mitotic index determination (colchicine metaphases per 1000 nuclei).
Main Results:
- A normal mitotic circadian curve for renocytes was established.
- All tested treatments (liver extract, plasma from intact mice, ES2 tumor extract, plasma from tumor-bearing mice) significantly reduced the mitotic activity of renal tubular cells compared to control groups.
- Mitotic activity values in treated animals were significantly lower than in controls.
Conclusions:
- The study demonstrates that liver extract, plasma from intact mice, ES2 tumor extract, and plasma from tumor-bearing mice inhibit the mitotic activity of renal tubular cells.
- This inhibition is likely mediated by non-specific plasmatic and/or tissue factors.