Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human colonic tumor cell kinetics: potential for therapy

S Lesher, L M Schiffer, M Phanse

    Cancer
    |November 1, 1977
    PubMed
    Summary

    New in vitro methods allow cell kinetic measurements in human colon tissues. Preliminary data show increased DNA synthesis in adenocarcinomas, suggesting potential response to S-phase drugs.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evidence for unstable periodic orbits in intact swimming lampreys, isolated spinal cords, and intermediate preparations.

    Annals of the New York Academy of Sciences·1999
    Same author

    Effect of dexamethasone on vascular function in RIF-1 tumors.

    Cancer research·1986
    Same author

    Optical monitoring of membrane potential: methods of multisite optical measurement.

    Society of General Physiologists series·1986
    Same author

    1H-NMR relaxation times and water compartmentalization in experimental tumor models.

    Magnetic resonance imaging·1986
    Same author

    Menstrual variation of normal breast NMR relaxation parameters.

    Journal of computer assisted tomography·1985
    Same author

    Preliminary observations on the correlation of proliferative phenomena with in vivo 31P NMR spectroscopy after tumor chemotherapy.

    Annals of the New York Academy of Sciences·1985

    Area of Science:

    • Oncology
    • Cell Biology
    • Gastroenterology

    Background:

    • Accurate cell kinetic measurements are crucial for understanding colorectal cancer progression and treatment response.
    • Traditional methods often require invasive procedures and multiple tissue samples.
    • Development of novel in vitro techniques offers a less invasive approach to assess tumor cell proliferation.

    Purpose of the Study:

    • To introduce and validate new in vitro cell kinetic measurement techniques for human colonic tissues.
    • To compare cell kinetic parameters in primary tumors, metastatic tumors, polyps, and normal bowel.
    • To investigate the relationship between cell kinetics and potential response to S-phase active chemotherapy.

    Main Methods:

    • Development of novel in vitro techniques for cell kinetic measurements.
    • Analysis of labeling index (LI), DNA synthesis time (Ts), and growth fraction (PDP) from single tissue samples.
    • Calculation of potential doubling time (Tc) for various colonic tissue types.

    Main Results:

    • Labeling index (LI) increases from low-grade polyps to higher adenocarcinomas.
    • Cell kinetics show minimal differences between primary and metastatic colorectal tumors.
    • Growth fraction (PDP) and DNA synthesis times (Ts) are largely consistent across tissue types, except for villous adenomas.

    Conclusions:

    • The new in vitro techniques provide reliable cell kinetic data without requiring in vivo methods.
    • Elevated labeling indices in a subset of primary adenocarcinomas suggest potential sensitivity to S-phase targeting drugs like 5-FU.
    • Further research is warranted to correlate these kinetic findings with clinical outcomes and treatment efficacy.

    Related Experiment Videos