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Related Experiment Videos

Evaluation of Ames' "Clini-Tek"

J D Peele, R H Gadsden, R Crews

    Clinical Chemistry
    |December 1, 1977
    PubMed
    Summary

    The Ames "Clini-Tek" instrument shows high reproducibility for reading N-Multistix dipsticks, particularly for glucose, ketone, urobilinogen, and nitrite. Proteinuria determination was highly accurate, while hemoglobinuria and bilirubinuria readings were less reproducible.

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    Area of Science:

    • Clinical Chemistry
    • Urinalysis Technology

    Background:

    • Semi-automated urinalysis instruments offer potential for standardized and efficient sample analysis.
    • N-Multistix dipsticks are widely used for qualitative and semi-quantitative urine analysis.

    Purpose of the Study:

    • To evaluate the reproducibility of reading N-Multistix dipsticks using the Ames Clini-Tek semi-automated urinalysis instrument.
    • To assess the accuracy of specific analyte determinations, including glucose, ketone, urobilinogen, nitrite, protein, hemoglobin, and bilirubin.

    Main Methods:

    • Artificial urine samples were prepared with predetermined analyte concentrations.
    • N-Multistix dipsticks were read using the Ames Clini-Tek instrument.
    • Reproducibility was assessed for multiple analytes, with a focus on false positive and false negative rates for proteinuria.

    Main Results:

    • High reproducibility (greater than 90%) was observed for glucose, ketone, urobilinogen, and nitrite.
    • Proteinuria determination demonstrated a low false positive rate (2-3%) and no false negatives.
    • Hemoglobinuria and bilirubinuria readings exhibited the lowest reproducibility.
    • Urobilinogen readings were not affected by bilirubin up to 32 mg/liter.
    • High precision was achieved with manufacturer-provided quality-control capsules.

    Conclusions:

    • The Ames Clini-Tek instrument provides reproducible results for several key analytes when using N-Multistix dipsticks.
    • While generally reliable, hemoglobinuria and bilirubinuria measurements require careful interpretation due to lower reproducibility.
    • The instrument demonstrates potential for accurate proteinuria screening.

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