Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrin stimulation regulates polymorphonuclear leukocytes inflammatory cytokine expression

H H Simms1, R D'Amico, K I Bland

  • 1Brown University School of Medicine/Rhode Island Hospital, Department of Surgery, Providence, USA.

Annals of Surgery
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interaction between tumor-infiltrating lymphocytes and BMI in early HER2-positive breast cancer: analysis of the ShortHER trial.

ESMO open·2025
Same author

Corrigendum to [Effects of a new compound containing Palmitoylethanolamide and Baicalein in myocardial ischaemia/reperfusion injury in vivo] [PHYMED: 2019 Feb 15;54:27-42 Epub 2018 Sep 19].

Phytomedicine : international journal of phytotherapy and phytopharmacology·2023
Same author

Impact of sarcopenia in SARS-CoV-2 patients during two different epidemic waves.

Clinical nutrition ESPEN·2022
Same author

Radiotherapy Quality Assurance in the PORTEC-3 (TROG 08.04) Trial.

Clinical oncology (Royal College of Radiologists (Great Britain))·2021
Same author

Serial cervical-length measurements after first episode of threatened preterm labor improve prediction of spontaneous delivery prior to 37 weeks' gestation.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2020
Same author

Diagnostic accuracy of a velcro sound detector (VECTOR) for interstitial lung disease in rheumatoid arthritis patients: the InSPIRAtE validation study (INterStitial pneumonia in rheumatoid ArThritis with an electronic device).

BMC pulmonary medicine·2019

Integrin binding regulates polymorphonuclear leukocyte (PMN) cytokine receptor expression. Specific integrins like CD49e and CD49f modulate TNF-alpha and IL-1 beta receptors, impacting inflammatory responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Beta 2 integrins are known to mediate polymorphonuclear leukocyte (PMN) adherence and chemotaxis.
  • The role of alpha 1-6/beta 1 integrins in regulating cytokine receptor expression on PMNs remains largely unexplored.

Purpose of the Study:

  • To investigate how integrin binding influences the expression of cytokine receptors on PMNs.
  • To elucidate the specific integrins involved in modulating tumor necrosis factor-alpha receptor (TNF-alpha R) and interleukin-1 beta receptor (IL-1 beta R) expression.

Main Methods:

  • Human PMNs were adhered to surfaces coated with fibronectin or laminin.
  • Receptor expression was quantified using radiolabeled monoclonal antibodies against TNF-alpha R and IL-1 beta R.
  • The involvement of specific beta 1 integrins (VLA-1 to VLA-6) and protein tyrosine kinase pathways was assessed using blocking antibodies and herbimycin A.

Related Experiment Videos

Main Results:

  • Adherence to fibronectin increased TNF-alpha R expression, while laminin adherence decreased IL-1 beta R expression.
  • VLA-5 (CD49e) mediated the fibronectin effect on TNF-alpha receptors, and VLA-6 (CD49f) mediated the laminin effect on IL-1 beta receptors.
  • Both integrin-mediated effects were dependent on protein tyrosine kinase activation, as shown by herbimycin A treatment.

Conclusions:

  • Integrin engagement distinctly regulates PMN cytokine receptor expression: CD49e upregulates TNF-alpha receptors, and CD49f downregulates IL-1 beta receptors.
  • These integrin-mediated modulations of cytokine receptor expression are dependent on protein tyrosine kinase signaling.
  • The observed changes in receptor expression correlate with functional activity, suggesting a mechanism for PMN participation in inflammatory responses via matrix protein integrin receptors.