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Demonstration of two disease specific antigens in circulating immune complexes
Clinical and Experimental Immunology
|September 1, 1979
Summary
This study introduces a new method to measure antigenic cross-reactivity in soluble immune complexes. Researchers found shared antigens in patients with haematuria and hypertension, and distinct antigens in bullous pemphigoid patients.
Area of Science:
- Immunology
- Clinical Chemistry
Background:
- Soluble immune complexes (SICs) play a role in various autoimmune diseases.
- Assessing antigenic similarities between SICs from different patient groups is crucial for understanding disease pathogenesis.
Purpose of the Study:
- To develop and validate a novel method for evaluating antigenic cross-reactivity between SICs.
- To investigate shared antigens in patients with haematuria and hypertension and bullous pemphigoid.
Main Methods:
- A new assay was developed using polyethylene glycol precipitation of sera.
- Immune complexes were dissociated, coated onto plastic cups, and radioiodinated complexes were added for reassociation.
- Antigenic cross-reactivity was quantified by measuring the binding of labeled complexes.
Main Results:
- The method successfully detected antigenic cross-reactivity between SICs.
- A common antigen was identified in SICs from patients with haematuria and hypertension.
- A distinct antigen was found in SICs from patients with bullous pemphigoid.
Conclusions:
- The described method is effective for assessing antigenic cross-reactivity in SICs.
- Specific antigens are associated with haematuria/hypertension and bullous pemphigoid, suggesting distinct immunopathogenic mechanisms.