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Updated: Aug 11, 2026

Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
Published on: November 29, 2011
Estrogen receptor mRNA in mineralized tissues of rainbow trout: calcium mobilization by estrogen
K J Armour1, D B Lehane, F Pakdel
1Department of Animal and Plant Sciences, University of Sheffield, UK. K.Armour@rri.sari.ac.uk
Abstract:
RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.
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