[A simple method for the radioimmunologic determination of sex steroids in plasma (author's transl)]

A simple method for the determination of plasma steroids by radioimmunoassay is described. Essential steps are: Dissolution of extraction residues in 20 microliter ethyl alcohol or direct addition of 20 microliter alcoholic plasma extract to the incubation tubes, addition of tracer solution with known tracer mass and antiserum dilution or gelatine phosphate buffer, incubation for 40 min and separation of free from bound radioactivity by charcoal-coated florisil. Plasma steroid levels are calculated by a simple equation, an immunologic equivalent to the tracer mass serves as the reference value.