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Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli
H R Kaback1, J Voss, J Wu
1Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024-1662, USA. RonaldK@HHMI.UCLA.edu
Current Opinion in Structural Biology
|August 1, 1997
Abstract:
Recent advances in protein engineering have facilitated the development of alternative approaches to determine helix packing in polytopic membrane proteins. Using the lac permease as a paradigm, several site-directed biophysical and biochemical techniques are described which should be generally applicable.