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Related Experiment Videos

Gene trapping and functional genomics

M J Evans1, M B Carlton, A P Russ

  • 1Institute of Cancer and Developmental Biology, University of Cambridge, UK. mje2@cam.ac.uk

Trends in Genetics : TIG
|September 1, 1997
PubMed
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Large-scale gene trapping in embryonic stem cells enables functional genomic analysis. This approach integrates gene discovery, mapping, and mutant phenotype observation for mammalian genomes.

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Classical genetics relies on random gene disruption, lacking structural information.
  • Modern genomics focuses on gene structure (mapping, sequencing) without functional insights.
  • Rapid gene discovery from genome projects necessitates new methods for functional analysis.

Purpose of the Study:

  • To develop an efficient, large-scale approach for functional genomic analysis.
  • To integrate gene discovery, mapping, expression patterns, and in vivo mutant phenotypes.
  • To enable the generation of mutants for a significant portion of the mammalian genome.

Main Methods:

  • Utilizing gene trapping in embryonic stem (ES) cells for insertional mutagenesis.
  • Developing an indexed library of mutated ES cell clones.

Related Experiment Videos

  • Combining gene discovery, mapping, expression analysis, and phenotype observation.

    • Main Results:

    • Gene trapping in ES cells provides an efficient method for functional analysis of the murine genome.
    • An indexed library of mutated ES cell clones integrates multiple aspects of genetic analysis.
    • The potential to generate mutants for a large proportion of mammalian genes is demonstrated.

    Conclusions:

    • Gene trapping in ES cells is a powerful strategy for large-scale functional genomics.
    • This integrated approach accelerates the understanding of gene function at multiple biological levels.
    • It is feasible to create comprehensive mutant collections for mammalian genome-wide functional studies.