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Isolated hepatocytes--past, present and future

M N Berry1, A R Grivell, M B Grivell

  • 1Department of Medical Biochemistry, School of Medicine, Faculty of Health Sciences, Flinders University of South Australia, Adelaide, Australia.

Cell Biology and Toxicology
|July 1, 1997
PubMed
Summary
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Isolated hepatocytes are crucial for studying drug metabolism and liver failure. Recent research shows energy transfer between cell compartments without ATP, advancing hepatocyte research.

Area of Science:

  • Hepatocyte isolation and cell biology research.

Background:

  • Early hepatocyte isolation methods (1950s-1960s) caused significant cell damage.
  • Collagenase-based perfusion techniques developed in 1967 enabled the preparation of intact isolated hepatocytes.
  • Isolated hepatocytes have become indispensable tools in biomedical research.

Purpose of the Study:

  • To review the historical development and applications of isolated hepatocyte preparations.
  • To highlight advances in culturing hepatocytes for drug metabolism and toxicology studies.
  • To present novel findings on intercellular energy transfer within hepatocytes.

Main Methods:

  • Historical review of hepatocyte isolation techniques.
  • Description of methods for maintaining differentiated cultured hepatocytes, including cytochrome P450 complexes.

Related Experiment Videos

  • Presentation of experimental evidence for long-range interactions and energy transfer between hepatocyte compartments.

    • Main Results:

    • Collagenase perfusion significantly improved hepatocyte viability and integrity.
    • Cultured hepatocytes are vital for investigating therapeutic agent metabolism and toxicology.
    • Evidence suggests energy transfer occurs between hepatocyte compartments independently of ATP turnover.

    Conclusions:

    • Isolated hepatocytes have revolutionized liver research, enabling studies previously not possible.
    • Improvements in hepatocyte culture techniques support advanced research in drug development and toxicology.
    • The discovery of non-ATP-dependent energy transfer opens new avenues for understanding hepatocyte function and disease.