Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Helper T-lymphocyte count. TRAx CD4 test kit versus conventional flow cytometry

A J Saah1, C Spruill, D R Hoover

  • 1Department of Epidemiology, Johns Hopkins School of Public Health, Baltimore, MD 21205, USA.

Archives of Pathology & Laboratory Medicine
|September 26, 1997
PubMed
Summary

The TRAx enzyme-linked immunosorbent assay is an acceptable method for measuring helper T-lymphocyte counts. Recalibration is recommended for improved accuracy below 200 cells/µL.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Frailty-Related Factors among Women Living with and without HIV Aged 40 Years and Older. The Women's Interagency HIV Study.

The Journal of frailty & aging·2024
Same author

Simple Power and Sample Size Estimation for Non-Randomized Longitudinal Difference in Differences Studies.

Journal of biometrics & biostatistics·2019
Same author

Longitudinal evaluation of markers of inflammation in HIV-positive and HIV-negative Rwandan women.

HIV medicine·2018
Same author

Bone quality assessed using quantitative ultrasound at the distal radius does not differ in antiretroviral therapy-naïve HIV-positive and HIV-negative Rwandan women.

HIV medicine·2016
Same author

Risk Factors for the Development of BK Virus Nephropathy in Renal Transplant Recipients.

Transplantation proceedings·2015
Same author

Efficacy of caspofungin as salvage therapy for invasive aspergillosis compared to standard therapy in a historical cohort.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2010

Area of Science:

  • Immunology
  • Clinical Diagnostics
  • Assay Development

Background:

  • Helper T-lymphocyte (CD4+) counts are critical for monitoring immune status, particularly in HIV infection.
  • Accurate enumeration of T-lymphocytes is essential for guiding therapeutic interventions.
  • Novel diagnostic assays require rigorous validation against established methods.

Purpose of the Study:

  • To evaluate the performance of a new enzyme-linked immunosorbent assay (ELISA) system, TRAx, for quantifying helper T-lymphocyte counts.
  • To compare the results obtained from the TRAx ELISA with those from flow cytometry, a standard method.

Main Methods:

  • A cohort of 111 human immunodeficiency virus-infected injection drug users was studied.
  • Helper T-lymphocyte counts were determined using both flow cytometry and duplicate runs of the TRAx ELISA.

Related Experiment Videos

  • Statistical analysis included correlation coefficients and cross-tabulation to assess agreement.

    • Main Results:

    • The TRAx ELISA demonstrated high correlation with flow cytometry (r = .93 and .91).
    • Agreement between TRAx and flow cytometry was substantial for counts >200 cells/µL (71-90%).
    • Performance at counts ≤200 cells/µL showed variability (41-73% agreement).

    Conclusions:

    • The TRAx ELISA system is a viable option for helper T-lymphocyte measurement.
    • Recalibration of the TRAx ELISA is necessary to enhance accuracy for low T-cell counts (<200 cells/µL).
    • Further optimization may improve its utility in clinical settings with low lymphocyte counts.