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Generation of Human Alloantigen-specific T Cells from Peripheral Blood
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[The unknown functions of heterologous antigens of different origins]

A I Nikolaeva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |May 1, 1997
    PubMed

    After a single injection of 125I-labeled erythrocytic antigen to mice, the protein determinants of this antigen were found in nuclear RNP of B lymphocytes of the immune animals. On day 2 after the injection of Shigella sonnei phase I LPS (also containing protein) to mice hybridization between total RNA isolated from B lymphocytes and 32P-labeled plasmid pSS 120, controlling the synthesis of the S. sonnei phase I antigen, was observed. On day 1 after immunization no hybridization between cell RNA and plasmid pSS 120 occurred. Besides, no hybridization of plasmid DNA with the RNA of nonimmune animals or with RNA, obtained on day 3 after the injection of Salmonella kentucky LPS to mice, was observed. The signal indicating the occurrence of hybridization between the RNA of immune B lymphocytes and plasmid pSS 120 appeared on day 2 and increased on days 3 and 5 after the injection of the antigen. This was seemingly indicative of the accumulation of new nucleotide sequences, complementary to the gene controlling the synthesis of the heterologous antigen which caused immune response, in immune B lymphocytes.

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