Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fas ligand expression and function in systemic lupus erythematosus

J McNally1, D H Yoo, J Drappa

  • 1Hospital for Special Surgery, Cornell University Medical Center, New York, NY 10021, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|October 31, 1997
PubMed
Summary

Systemic lupus erythematosus (SLE) patients do not show specific defects in Fas ligand (FasL) function. T cell cytotoxicity reductions in SLE patients are comparable to rheumatoid arthritis patients, suggesting subtle activation differences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arterial Spin-Labeling MR Imaging for the Differential Diagnosis of Venous-Predominant AVMs and Developmental Venous Anomalies.

AJNR. American journal of neuroradiology·2023
Same author

Suspected Metallic Embolization Distal to Coiled Intracranial Aneurysms Detectable by Susceptibility-Weighted MR Imaging.

AJNR. American journal of neuroradiology·2020
Same author

Contralateral Approach to Coil Embolization of Proximal A1 Aneurysms Using the Anterior Communicating Artery.

AJNR. American journal of neuroradiology·2018
Same author

Comparison of Clinical Outcomes of Intracranial Aneurysms: Procedural Rupture versus Spontaneous Rupture.

AJNR. American journal of neuroradiology·2017
Same author

Risk Factor Analysis of Recanalization Timing in Coiled Aneurysms: Early versus Late Recanalization.

AJNR. American journal of neuroradiology·2017
Same author

Fate of Coiled Aneurysms with Minor Recanalization at 6 Months: Rate of Progression to Further Recanalization and Related Risk Factors.

AJNR. American journal of neuroradiology·2016

Area of Science:

  • Immunology
  • Autoimmune Diseases
  • Cellular Biology

Background:

  • Mutations in Fas receptor or Fas ligand (FasL) are linked to autoimmune diseases like lupus.
  • Systemic lupus erythematosus (SLE) is a chronic autoimmune disease affecting multiple organs.

Purpose of the Study:

  • To investigate if patients with SLE exhibit impaired FasL function.
  • To compare T cell effector functions in SLE patients versus healthy controls.

Main Methods:

  • Compared Fas expression and apoptosis induction in superantigen-activated CD4+ T cells from SLE patients and controls.
  • Assessed T cell cytotoxicity using anti-CD3/IL-2 activated T cells, examining both Fas-mediated and perforin/granzyme pathways.
  • Utilized Fas-sensitive and -negative targets and concanamycin blockade to differentiate cytotoxic mechanisms.

Related Experiment Videos

Main Results:

  • No significant differences in Fas expression or Fas-mediated apoptosis were found between SLE and control CD4+ T cells.
  • SLE T cells showed a modest reduction in cytotoxicity (-8%) compared to controls, similar to rheumatoid arthritis patients.
  • Reductions in cytotoxicity were observed in both Fas and perforin/granzyme pathways, indicating no specific defect in FasL function.

Conclusions:

  • The majority of SLE patients do not exhibit specific defects in FasL function under tested in vitro conditions.
  • Observed reductions in T cell cytotoxicity in SLE and rheumatoid arthritis patients likely stem from subtle differences in T cell activation.
  • Further research may explore these activation differences in patient-derived T cells.