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Conformational fluctuations in single DNA molecules

S Wennmalm1, L Edman, R Rigler

  • 1Department of Medical Biophysics, Karolinska Institute, S-171 77, Stockholm, Sweden.

Proceedings of the National Academy of Sciences of the United States of America
|October 6, 1997
PubMed
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Single DNA molecules exhibit conformational fluctuations, transitioning between states. This behavior, observed using fluorescence, suggests a distribution of transition rates, similar to ion channels.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Physical Chemistry

Background:

  • Fluorescent lifetimes of dye-tagged DNA molecules indicate distinct molecular conformations.
  • Conformational fluctuations in DNA can be studied using fluorescence correlation spectroscopy.
  • Observed relaxation behavior suggests a distribution of transition rates between DNA conformations.

Purpose of the Study:

  • To investigate conformational fluctuations in single DNA molecules.
  • To determine if the observed distribution of transition rates is homogeneous or inhomogeneous.
  • To characterize the dynamics of DNA conformational changes.

Main Methods:

  • Utilized a tetramethylrhodamine-linked 217-bp DNA oligonucleotide as a probe.
  • Employed single-molecule fluorescence measurements.

Related Experiment Videos

  • Attached DNA molecules to a streptavidin-coated surface for observation.

    • Main Results:

    • Directly observed fluorescence fluctuations corresponding to transitions between two conformational states in single DNA molecules.
    • Demonstrated that these conformational fluctuations are characteristic of individual molecules.
    • Found similarities between DNA conformational dynamics and those observed in single ion channels.

    Conclusions:

    • Single DNA molecules exhibit dynamic conformational changes.
    • The observed dynamics are consistent with a distribution of transition rates between conformational states.
    • The study provides insights into the molecular mechanisms governing DNA conformational flexibility.