Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

U-insertion/deletion Edited Sequence Database

L Simpson1, S H Wang, O H Thiemann

  • 1Howard Hughes Medical Institute, Department of Molecular, Cell and Developmental Biology, UCLA, Los Angeles, CA 90095-1662, USA. simpson@hhmi.ucla.edu

Nucleic Acids Research
|February 21, 1998
PubMed
Summary

This study details the U-insertion/deletion Edited Sequence Database, a resource for mitochondrial RNA editing in kinetoplastids. It compiles gene sequences and editing information, aiding research into this unique post-transcriptional modification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Scoping Review to Map Health Outcomes in Inducible Laryngeal Obstruction.

International journal of language & communication disorders·2025
Same author

Factors associated with emergency admission for people dying from cancer in Northern Ireland: an observational data linkage study.

BMC health services research·2023
Same author

The challenge of providing medical follow-up for sexual assault victims: can we predict who will attend? A retrospective cross-sectional study.

Sexual health·2023
Same author

Lip symmetry following rotation advancement cleft lip repair in 5-year-old children treated by Ralph Millard and Ron Pigott.

JPRAS open·2022
Same author

Infectious mononucleosis - should we routinely assess liver function in acute presentation and follow up?

The Journal of laryngology and otology·2022
Same author

Pregnancies in women with Turner syndrome: a retrospective multicentre UK study.

BJOG : an international journal of obstetrics and gynaecology·2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Uridine insertion/deletion RNA editing is a unique post-transcriptional modification found in the mitochondria of kinetoplastid protozoa.
  • This process is crucial for generating functional mRNAs in these organisms.

Purpose of the Study:

  • To create and present the U-insertion/deletion Edited Sequence Database, a centralized repository for mitochondrial gene and edited mRNA sequences.
  • To facilitate research on RNA editing in kinetoplastids by providing comprehensive sequence data and analysis tools.

Main Methods:

  • Compilation of mitochondrial DNA, unedited and edited mRNA, and predicted protein sequences from five kinetoplastid species.
  • Generation of sequence alignments for key species (Leishmania tarentolae, Trypanosoma brucei) and creation of 'map' files detailing editing sites.

Related Experiment Videos

  • Development of genomic maps with clickable genes and inclusion of aligned nuclear rRNA sequences for phylogenetic analysis.

    • Main Results:

    • The database includes diverse sequence data (DNA, mRNA, protein) in GCG format for five kinetoplastid species.
    • Detailed 'map' files illustrate U-insertion/deletion sites and their impact on amino acid sequences.
    • Genomic maps and phylogenetic data on nuclear rRNA sequences are provided to contextualize RNA editing.

    Conclusions:

    • The U-insertion/deletion Edited Sequence Database serves as a valuable resource for studying mitochondrial RNA editing in kinetoplastids.
    • The database supports research into the mechanisms and evolutionary origins of this fundamental biological process.