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Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper
S A Fiscus1, D Brambilla, L Grosso
1Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 27599-7140, USA. sfiscus@email.unc.edu
Journal of Clinical Microbiology
|February 12, 1998
Abstract:
We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity and reproducibility.
