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Low-dose automated electron tomography: a recent implementation
B K Rath1, M Marko, M Radermacher
1Wadsworth Center, New York State Department of Health, Albany, New York 12201-0509, USA.
Journal of Structural Biology
|January 27, 1998
Abstract:
Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made.