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Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica
X Fan1, J R McLachlin, R F Weaver
1Department of Biochemistry, Cell and Molecular Biology, University of Kansas, Lawrence 66045-2106, USA.
Virology
|February 10, 1998
Abstract:
We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa protein and appears to stimulate the activity of the viral protein kinase-1 in vitro. Northern blotting analysis revealed that pkip is a late gene. Attempts to plaque purify a pkip- virus were unsuccessful.