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Peak broadening in capillary zone electrophoresis
1Faculty of Science, Charles University, Prague, Czech Republic. gas@mail.natur.cuni.cz
Electrophoresis
|February 10, 1998
Summary
This review details factors causing peak broadening in capillary zone electrophoresis (CZE) in free solutions. Understanding these dispersion effects is crucial for optimizing CZE separation efficiency.
Area of Science:
- Analytical Chemistry
- Separation Science
- Electrophoresis
Background:
- Capillary Zone Electrophoresis (CZE) is a powerful separation technique.
- Peak broadening in CZE reduces separation efficiency and resolution.
- Understanding dispersion sources is critical for method development.
Purpose of the Study:
- To review literature on peak broadening in free solution CZE.
- To identify and describe key contributors to peak dispersion.
- To cover publications from 1992 to early 1997.
Main Methods:
- Literature review of 71 publications.
- Description of extracolumn effects (injection, detector aperture).
- Analysis of longitudinal diffusion, Joule heating, and electromigration dispersion.
Main Results:
- Detailed discussion of factors like path length differences, wall adsorption, and flow effects.
- Explanation of homogeneous and nonhomogeneous electroosmotic flow impacts.
- Presentation of peak dispersion via plate-height model or continuity equation solutions.
Conclusions:
- Peak broadening in CZE is multifactorial.
- Extracolumn effects and intrinsic CZE phenomena contribute significantly.
- Mathematical models aid in understanding and mitigating dispersion for improved CZE performance.