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Functional analysis of the yeast genome
1Department of Biochemistry, Stanford University School of Medicine, California 94305-5307, USA. winzeler@cmgm.stanford.edu
Current Opinion in Genetics & Development
|February 20, 1998
Summary
The yeast Saccharomyces cerevisiae genome sequence enables functional genomics. Researchers are assigning biological functions to over 6,000 yeast genes using innovative methods.
Area of Science:
- Genomics
- Molecular Biology
- Yeast Research
Background:
- The completion of the Saccharomyces cerevisiae genome sequence marks a significant advancement.
- Assigning biological functions to all yeast genes is a major challenge in genomics.
Purpose of the Study:
- To determine the biological function of each of the over 6,000 open reading frames in the yeast genome.
- To leverage genomic sequence data for comprehensive functional analysis.
Main Methods:
- Development of innovative approaches to exploit yeast sequence data.
- Application of high-throughput methods for genome-wide analysis.
Main Results:
- Generation of data on gene expression levels for the entire yeast genome.
- Determination of protein levels and subcellular localization for yeast genes.
- Assignment of biological functions to yeast open reading frames.
Conclusions:
- The yeast genome sequence facilitates a new era of functional genomics.
- Innovative strategies enable comprehensive functional annotation of a complete genome.