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False negative HLA-DR assignment necrokidney donors. A glucocorticoid effect?
Tissue Antigens
|October 1, 1979
Summary
Human Leukocyte Antigen (HLA)-DR typing of potential kidney donors using peripheral blood B lymphocytes (PBBL) can yield false-negative results. Spleen-derived B lymphocytes offer a reliable alternative for accurate HLA-DR antigen assignment in such cases.
Area of Science:
- Immunogenetics
- Transplantation immunology
Background:
- Accurate Human Leukocyte Antigen (HLA) typing is crucial for kidney transplantation success.
- Peripheral blood B lymphocytes (PBBL) are commonly used for HLA-DR typing.
Observation:
- Thirteen potential necrokidney donors underwent HLA-DR typing using PBBL.
- Antigen assignment was unsuccessful in 10 donors due to non-reactivity of PBBL, even with positive controls.
- In five of these donors, HLA-DR typing was repeated using B lymphocytes from spleen tissue.
Findings:
- B lymphocytes isolated from spleen tissue showed strong, specific reactions, enabling reliable HLA-DR antigen assignment.
- The non-reactive results from PBBL were identified as 'falsely negative'.
- Glucocorticoid treatment prior to blood sampling is hypothesized as the cause of this phenomenon.
Implications:
- This study highlights potential pitfalls in HLA-DR typing using PBBL.
- Spleen-derived B lymphocytes may serve as a more reliable source for HLA-DR typing in specific clinical scenarios.
- Investigating the impact of glucocorticoid treatment on HLA-DR typing accuracy is warranted.