Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BRCA1 regulates p53-dependent gene expression

T Ouchi1, A N Monteiro, A August

  • 1Laboratory of Molecular Oncology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Randomized Clinical Trial Evaluating Lactiplantibacillus Plantarum for the Prevention of GI aGvHD: A Report From the Children's Oncology Group (ACCL1633).

Transplantation and cellular therapy·2025
Same author

The Lifebox Surgical Headlight Project: engineering, testing, and field assessment in a resource-constrained setting.

The British journal of surgery·2020
Same author

The decidua of preeclamptic-like BPH/5 mice exhibits an exaggerated inflammatory response during early pregnancy.

Journal of reproductive immunology·2017
Same author

HUS1 regulates in vivo responses to genotoxic chemotherapies.

Oncogene·2015
Same author

In situ protein expression of RRM1, ERCC1, and BRCA1 in metastatic breast cancer patients treated with gemcitabine-based chemotherapy.

Cancer investigation·2009
Same author

Three-color intranuclear staining for measuring mitosis and apoptosis in cells transfected with a GFP-tagged histone.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2009
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

BRCA1 (Breast Cancer gene 1) acts as a coactivator for p53, enhancing its transcriptional activity. This interaction is crucial for tumor suppression and involves the p53-responsive elements and BRCA1

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Mutations in BRCA1 (Breast Cancer gene 1) are linked to hereditary breast and ovarian cancers.
  • The tumor suppressor protein p53 plays a critical role in cellular responses to DNA damage.

Purpose of the Study:

  • To investigate the functional relationship between BRCA1 and p53.
  • To determine if BRCA1 influences the transcriptional activity of p53.

Main Methods:

  • Assessed BRCA1's effect on p53-responsive promoter constructs in various mouse fibroblast models (including p53 knockout).
  • Utilized mutant BRCA1 lacking the BRCT domain.
  • Performed coimmunoprecipitation assays to detect protein-protein interactions.

Main Results:

Related Experiment Videos

  • BRCA1 stimulates artificial and genomic promoters containing p53-responsive elements.
  • This stimulation is dependent on wild-type p53 and is reduced with BRCA1 mutants lacking the BRCT domain.
  • BRCA1 and p53 were found to coimmunoprecipitate, indicating a physical interaction.

Conclusions:

  • BRCA1 functions as a coactivator for p53.
  • This suggests a novel mechanism for BRCA1 in tumor suppression through its interaction with the p53 pathway.