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Cadaveric bone marrow and spleen cells for transplantation
G Söderdahl1, C Tammik, M Remberger
1Department of Transplantation Surgery, Huddinge Hospital, Sweden.
Bone Marrow Transplantation
|March 5, 1998
Summary
Cadaveric bone marrow from vertebrae yields significantly more nucleated cells for transplantation than sternum or ribs. This research confirms the viability and functionality of stem cells from deceased donors for potential therapeutic use.
Area of Science:
- Hematology
- Transplantation Immunology
- Regenerative Medicine
Background:
- Bone marrow transplantation is a critical therapy for various hematologic malignancies and disorders.
- Cadaveric bone marrow procurement presents a potential alternative source of hematopoietic stem cells, but optimal harvesting and processing methods require investigation.
- Ensuring the viability and functional capacity of stem cells from deceased donors is paramount for successful transplantation.
Purpose of the Study:
- To determine optimal conditions for harvesting and procuring cadaveric bone marrow for transplantation.
- To compare cell yields and viability from different anatomical sites (vertebrae, sternum, ribs) and spleen.
- To evaluate the efficacy of T-cell depletion methods on stem cell quality and quantity.
Main Methods:
- Bone marrow was harvested from 20 brain-dead donors; spleen cells were obtained from three donors.
- Nucleated cell counts and viability were assessed from different anatomical sources.
- Magnetic bead-based T-lymphocyte depletion and CD34-positive cell selection were performed.
- Colony-forming unit assays (CFU-GM, BFU-E, CFU-GEMM) were used to evaluate stem cell function.
Main Results:
- Vertebrae yielded significantly higher nucleated cell counts compared to sternum and ribs.
- Cell viability was unaffected by storage temperature (4°C or 20°C) or duration (6-72 hours).
- Magnetic bead T-cell depletion removed 96% of T lymphocytes without affecting total stem cell yield; CD34 selection achieved 99.6% depletion but with a 60% loss of CD34+ cells.
- Cadaveric bone marrow demonstrated comparable colony-forming unit potential to bone marrow from living donors, while spleen cells showed significantly fewer CFUs.
Conclusions:
- Cadaveric bone marrow can be harvested and procured with high viability and good function.
- Vertebrae are the optimal source for nucleated cells from cadaveric bone marrow.
- Optimized harvesting and procurement techniques can yield sufficient stem cells for transplantation from deceased donors.