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[CD5-positive B lymphocytes and antinuclear antibodies]
T Fucíková1, J Cermáková, H Marecková
1Ustav klinické imunologie 1, LF UK, Praha.
Casopis Lekaru Ceskych
|March 25, 1998
Summary
The amount of CD5 positive B lymphocytes in blood does not correlate with the presence of antinuclear antibodies (ANA). This study found no direct link between these markers in individuals with various diseases or autoimmune conditions.
Area of Science:
- Immunology
- Autoimmunity
Background:
- Natural autoantibodies, including antinuclear antibodies (ANA), are produced by CD5-expressing lymphocytes.
- The precise role of CD5-positive B lymphocytes in autoimmunity remains unclear.
Purpose of the Study:
- To investigate the relationship between the proportion of CD5-positive B lymphocytes in peripheral blood and the presence of ANA.
- To explore potential associations in subjects with different diseases and autoimmune conditions.
Main Methods:
- Flow cytometry was used to quantify CD5-positive lymphocytes with anti-CD5 and anti-CD19 antibodies.
- Indirect immunofluorescence detected ANA (IgG, IgA, IgM) using human leukocytes and HEP-2 cells as substrates.
Main Results:
- No direct correlation was found between CD5-positive B lymphocyte levels and ANA presence in 134 subjects, including 33 with autoimmune diseases.
- ANA IgM predominated in patients with recurrent respiratory infections, while IgG predominated in systemic diseases.
- ANA IgG decreased and IgM increased after successful immunosuppressive treatment. ANA IgM was more prevalent in healthy individuals over 60 and in women.
Conclusions:
- The quantity of CD5-positive B lymphocytes in peripheral blood is not associated with the presence of antinuclear antibodies.
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