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Frequency shift analysis of tetracycline
S A Nabi1, E S Abu-Nameh, M I Helaleh
1Department of Chemistry, Aligarh Muslim University, India.
Acta Poloniae Pharmaceutica
|May 1, 1997
Summary
This study introduces a new spectrophotometric method for quantifying tetracycline using DMSO-induced wavelength shifts. The method accurately measures tetracycline in water and spiked urine samples at low concentrations.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Pharmaceutical Analysis
Background:
- Tetracycline is a widely used antibiotic.
- Accurate quantification of tetracycline is crucial for therapeutic drug monitoring and quality control.
- Existing analytical methods may require complex procedures or expensive equipment.
Purpose of the Study:
- To develop a simple and sensitive spectrophotometric method for tetracycline determination.
- To investigate the interaction between tetracycline and DMSO for analytical purposes.
- To validate the method for real-world sample matrices like urine.
Main Methods:
- Spectrophotometric analysis of tetracycline in aqueous solutions.
- Utilizing the wavelength shift of absorption maxima (λmax 360 nm) caused by hydrogen bonding with DMSO.
- Quantification within the concentration range of 0.04 to 0.18 ppm.
Main Results:
- A distinct wavelength shift in tetracycline's absorption maximum was observed due to DMSO.
- The developed spectrophotometric method demonstrated good sensitivity for tetracycline detection.
- The method was successfully applied to determine tetracycline in spiked human urine samples.
Conclusions:
- The described spectrophotometric method offers a reliable approach for tetracycline quantification.
- The interaction with DMSO provides a basis for a straightforward analytical technique.
- The method's applicability to urine samples highlights its potential for clinical and forensic analysis.