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Use of flow cytometry as a tool to study mitochondrial membrane potential in isolated, living hepatocytes
S Salvioli1, R Maseroli, T L Pazienza
1Department of Biomedical Sciences, Section of General Pathology, University of Modena, via Campi, 287, 41100 Modena, Italy. bobyleva@unimo.it
Biochemistry. Biokhimiia
|June 2, 1998
Abstract:
The present paper describes the possibility of determination of mitochondrial membrane potential (Deltapsi) in isolated hepatocytes making use of a Deltapsi-sensitive dye, i.e., the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3, 3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and of cytofluorimetry. The validity of the method was proved by treating hepatocytes with FCCP (decrease of Deltapsi) and subsequent addition of 6-ketocholestanol (increase of Deltapsi). The results indicate that the proposed method may be used in laboratory practice.