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Clathrin gene expression is androgen regulated in the prostate
1Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905, USA.
Endocrinology
|April 7, 1998
Summary
Androgens regulate clathrin gene expression in prostate cells, impacting protein secretion. This suggests clathrin
Area of Science:
- Molecular Endocrinology
- Cell Biology
- Prostate Cancer Research
Background:
- Androgens are crucial for prostate development and function.
- Androgen-regulated proteins like PSA and HGK are secreted into prostatic fluid.
- Novel androgen-regulated genes in prostate cancer are of significant interest.
Purpose of the Study:
- To investigate the role of androgens in regulating clathrin gene expression in the prostate.
- To determine if clathrin is involved in the secretion of androgen-regulated proteins.
Main Methods:
- Differential display PCR to identify androgen-regulated genes.
- Analysis of clathrin heavy and light chain mRNA and protein levels in LNCaP cells and rat prostates.
- Time- and concentration-dependent studies of androgen effects.
Main Results:
- Clathrin heavy chain mRNA and protein expression are up-regulated by androgens in LNCaP cells and rat prostates.
- Androgens also increase steady-state levels of clathrin light chains a and b.
- Androgen stimulation enhances translation of clathrin heavy chain mRNA.
Conclusions:
- Clathrin heavy and light chain genes are androgen-regulated in prostate cells.
- Clathrin may play a role in the exocytosis of key androgen-regulated secretory proteins.
- Further research into clathrin's function in prostate physiology and pathology is warranted.