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Scanning electron microscopy of dental calculus
Calcified Tissue Research
|August 3, 1976
Summary
Scanning electron microscopy revealed dental calculus comprises two calcification patterns: microbial plaque matrix calcification and larger crystal growths. These distinct patterns offer insights into calculus formation and composition.
Area of Science:
- Dental Morphology
- Biomineralization
- Microbiology
Background:
- Dental calculus, a calcified deposit on teeth, significantly impacts oral health.
- Understanding its structure is crucial for developing effective prevention and treatment strategies.
Purpose of the Study:
- To investigate the morphologic structure of anorganic dental calculus using scanning electron microscopy (SEM).
- To differentiate and characterize the components and calcification patterns within dental calculus.
Main Methods:
- Surface analysis of anorganic dental calculus using scanning electron microscopy (SEM).
- Comparison of observed patterns with transmission electron microscopy (TEM) findings and established mineralization types.
Main Results:
- Calculus exhibits two distinct calcification patterns: one associated with microorganisms (Type A) and another with larger crystal growths (Type B).
- Type A calcification shows sponge-like structures with voids from degenerated microorganisms.
- Type B calcification features larger, varied crystal growths, potentially representing different calcium phosphates.
Conclusions:
- Dental calculus is composed of at least two distinct mineralizing components.
- SEM surface observations provide valuable insights into the microstructural organization of dental calculus.
- Further analysis may confirm Type B crystals as octacalcium phosphate, whitlockite, or brushite.