Genetically modified mice for the study of apolipoprotein B

Area of Science:

• Biochemistry
• Genetics
• Metabolic Diseases

Background:

• Apolipoprotein B (apoB) plays a crucial role in lipoprotein metabolism and is implicated in various diseases.
• Understanding apoB's function requires effective model systems to study its complex roles.

Purpose of the Study:

• To review the advancements in studying apolipoprotein B (apoB) using transgenic and gene-targeted mouse models.
• To highlight how these models have facilitated research into atherogenesis, gene expression, and metabolic functions.

Main Methods:

• Utilized a variety of transgenic and gene-targeted mouse models.
• Conducted studies focusing on apoB structure/function, gene expression, and developmental roles.

Main Results:

• Generated novel mouse models for studying genetic and environmental factors in atherogenesis.
• Elucidated the regulation of apoB gene expression in the intestine and its role in embryonic development.
• Provided insights into the physiological basis for apoB-48 and apoB-100 isoforms and human hypobetalipoproteinemia.

Conclusions:

• Transgenic mouse models are invaluable tools for investigating apoB's multifaceted roles in health and disease.
• These models have significantly advanced our understanding of lipoprotein metabolism and related disorders.