Related Experiment Videos
Preparation of DNA-peptide conjugate using oxime resin
M Fujii1, T Hasegawa, I Koujima
1Department of Industrial Chemistry, Faculty of Engineering in Kyushu, Kinki University, Fukuoka, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
In order to prepare oligonucleotide-peptide conjugate molecules bearing additional functions, such as cellular membrane permeability, nuclease resistance, which are indispensable to antisense and triplex oligonucleotides for their practical use in vivo, it is found that application of oxime resin (Kaiser resin) to automated DNA synthesis and succeeding cleavage of DNA fragment bound to oxime resin by various nucleophiles, such as amines, alkoxides and protected peptides, give DNA conjugate molecules conventionally in moderate to good yields.