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RNA aptamers to a protease, subtilisin
H Takeno1, T Tanaka, Y Kikuchi
1Department of Ecological Engineering, Toyohashi University of Technology, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
RNA ligands (RNA aptamers) to a protease subtilisin were selected from pools of random RNA using SELEX (Systematic evolution of ligands by exponential enrichment). We selected RNA aptamers using a subtilisin-immobilized Sepharose column. After several rounds of selection, we obtained RNA aptamers that specifically inhibit the subtilisin activity. Characterization of the RNA aptamers will be reported.