Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lineage analysis using retroviral vectors

C L Cepko1, E Ryder, C Austin

  • 1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

Methods (San Diego, Calif.)
|June 3, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalized external aortic root support: The Dutch experience.

The Journal of thoracic and cardiovascular surgery·2025
Same author

Novel application of metabolic imaging of early embryos using a light-sheet on-a-chip device: a proof-of-concept study.

Human reproduction (Oxford, England)·2024
Same author

Genome Wide Conditional Mouse Knockout Resources.

Drug discovery today. Disease models·2024
Same author

Reply: Artificial intelligence as a door opener for a new era of human reproduction.

Human reproduction open·2023
Same author

Nanometer flat blazed x-ray gratings using ion beam figure correction.

Optics express·2023
Same author

Embryo selection through artificial intelligence versus embryologists: a systematic review.

Human reproduction open·2023
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Understanding cell lineage during development reveals crucial timing and location of developmental decisions. This study details using retroviral vectors to trace cell progeny in developing vertebrate tissues.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Cellular genealogy provides insights into developmental processes.
  • Tracing cell lineage is essential for understanding tissue development.
  • Various methods exist for marking progenitor cells and their progeny.

Purpose of the Study:

  • To describe the use of replication-incompetent retroviral vectors for analyzing lineal relationships in developing vertebrate tissues.
  • To provide an overview of the retroviral life cycle relevant to lineage tracing.
  • To detail the strategies and methods employed in the laboratory for this analysis.

Main Methods:

  • Utilizing replication-incompetent retroviral vectors for cell marking.
  • Following the progeny of marked progenitor cells.

Related Experiment Videos

  • Analyzing lineal relationships in developing vertebrate tissues.

    • Main Results:

    • Demonstrated the utility of retroviral vectors for tracing cell lineage.
    • Provided a comprehensive description of laboratory-specific methods.
    • Established a framework for understanding developmental decisions through cell genealogy.

    Conclusions:

    • Retroviral vectors are effective tools for studying cell lineage in vertebrate development.
    • Understanding cell genealogy aids in pinpointing critical developmental decision points.
    • The described methods offer valuable insights into developmental biology.