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Determination of the recognition sequence of the type II restriction endonuclease, LlaCI, from Lactococcus lactis W15
J Josephsen1, B Jørgen-Jensen, N R Nyengaard
1Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. jyj@kvl.dk
FEMS Microbiology Letters
|June 19, 1998
Abstract:
A new type II restriction endonuclease, called LlaCI, was partially purified from Lactococcus lactis subsp. cremoris W15. The characterisation of the LlaCI endonuclease showed it to be an isoschizomer of HindIII, recognising the sequence 5-'A decreases AGCTT-3'. The cleavage site is indicated by the arrow.