Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin stacking for capillary electrophoresis

Z K Shihabi1, M Friedberg

  • 1Pathology Department, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.

Journal of Chromatography. A
|July 1, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fetal cardiac filling and ejection time fractions by pulsed-wave Doppler: reference ranges and potential clinical application.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2020
Same author

Analysis and classification of serum cryoglobulins.

Methods in molecular medicine·2011
Same author

Myoglobin analysis.

Methods in molecular medicine·2011
Same author

Enzyme analysis : cathepsin d as an example.

Methods in molecular medicine·2011
Same author

Serum lamotrigine analysis.

Methods in molecular medicine·2011
Same author

Acetonitrile stacking : serum phenobarbital as an example.

Methods in molecular medicine·2011

Simple sample treatments, including high salt or acetonitrile, significantly enhance human insulin detection in capillary electrophoresis (CE) by inducing stacking. Acetonitrile treatment also removes interfering proteins, improving signal and enabling detection in tissue samples.

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Capillary electrophoresis (CE) suffers from poor detection limits for analytes like human insulin.
  • Effective stacking methods are crucial for improving sensitivity in CE separations.

Purpose of the Study:

  • To investigate simple sample pretreatment methods for enhancing human insulin detection in CE.
  • To evaluate the efficacy of dilute buffers, high salt content, and acetonitrile for inducing stacking.

Main Methods:

  • Human insulin samples were treated with dilute buffers, high salt concentrations, or 66% acetonitrile.
  • Capillary electrophoresis (CE) was used for separation and detection.
  • High-performance liquid chromatography (HPLC) and immunoassays were employed for confirmation.

Related Experiment Videos

Main Results:

  • High salt and acetonitrile treatments resulted in approximately 20-fold stacking, significantly improving detection limits.
  • Acetonitrile treatment effectively removed excess proteins and allowed for larger sample loading.
  • Insulin integrity was maintained in 66% acetonitrile, confirmed by HPLC and immunoassays.

Conclusions:

  • Acetonitrile treatment is a highly effective method for stacking human insulin in CE, enhancing sensitivity and enabling detection.
  • This approach facilitates the detection of insulin in complex biological matrices like pancreatic tissue homogenates.