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Evaluation of taurine metabolism in cats by dual stable isotope analysis
L B Fay1, S Métairon, F Montigon
1Nestlé Research Centre (Friskies Research), Nestec Ltd., Lausanne, Switzerland. laurent.fay@chlsnr.nestrd.ch
Abstract:
Taurine kinetics in cats was investigated using a bolus dose of [15N]- and [1,2-13C2]taurine. The comparison of [15N]- and [13C2]taurine kinetics permitted an evaluation of the extent of taurine transamination. A methodology which involves N-pentafluorobenzoyl di-n-butylamine derivatization of taurine and GC/MS measurements of the 15N- and 13C-enrichments in cat urine was developed. Accuracy of the measurements was determined using pure standard compounds and the results showed that [13C2]taurine does not interfere with [15N]taurine. In cats, no differences were observed between both tracers. Therefore, we conclude that taurine reversible transamination does not occur at a significant level in cats.