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Updated: Aug 10, 2026
12:04
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity
K L Gould1, C G Burns, A Feoktistova
1Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 38232, USA. kathy.gould@mcmail.vanderbilt.edu
Genetics
|July 3, 1998
Summary
The Schizosaccharomyces pombe cdc24(+) gene is essential for DNA replication and maintaining genomic integrity. Its loss causes S phase arrest, viability loss, and chromosome breakage.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- The cdc24(+) gene in Schizosaccharomyces pombe was previously identified as essential for S phase.
- A new temperature-sensitive allele, cdc24-G1, was isolated.
Purpose of the Study:
- To isolate and characterize the essential cdc24(+) gene.
- To elucidate the role of Cdc24p in DNA replication and genomic stability.
Main Methods:
- Gene isolation by complementation.
- DNA sequencing to predict protein structure.
- Homologous recombination to generate a null mutant.
- Cell cycle analysis and viability assays.
Main Results:
- The cdc24(+) gene encodes a 58 kD protein and is essential for cell viability.
- Loss of cdc24(+) function leads to a checkpoint-dependent S phase arrest with 2N DNA content.
- Cells lacking cdc24(+) exhibit rapid viability loss and chromosome breakage.
- An S. pombe homolog of DNA2 suppresses the cdc24 mutation.
Conclusions:
- Cdc24p is crucial for the progression of DNA replication.
- Cdc24p is required for maintaining genomic integrity during the cell cycle.
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