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Pulsed-field gel electrophoresis
1MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland. johnm@hgu.mrc.ac.uk
Molecular Biotechnology
|July 11, 1998
Abstract:
Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive description of the methodology. Established and novel applications are explored and the reader is provided with an extensive list of references.