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Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display. A
1Department of Tumor Molecular Biology, Institute of Basic Medical Sciences, Beijing, P.R. China.
Molecular Biotechnology
|July 11, 1998
Abstract:
Methods for retrieving and reamplifying the differentially expressed cDNA bands have been modified. Direct reamplification of differentially expressed bands after cutting from a polyacrylamide gel (PAG) followed by a simple rinse and crush step has proved to be more convenient and effective than the traditional glycogen-precipitation method. Combination of 30 cycles of differential display (DD) polymerase chain reaction (PCR) and 20 cycles of standard PCR reaction also yielded higher reamplification rates.