Related Experiment Video
Updated: Jul 29, 2026

11:25
Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Simultaneous estimation of tribenuron-methyl and its major metabolites by high-performance liquid chromatography
1Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi, India.
Biomedical Chromatography : BMC
|July 17, 1998
Abstract:
A rapid reversed phase HPLC method was developed for the separation of tribenuron-methyl and its three major metabolites (methyl-2-aminosulphonyl benzoate, 4-methoxy-6-methyl-2-methylamino-1,3,5-triazine and saccharin) using RP-18 column and methanol-water (60:40, v/v) as mobile phase. The limit of detection of all the four compounds was 0.01 ppm.

