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Skeletal myogenesis on elastomeric substrates: implications for tissue engineering

M M Mulder1, R W Hitchcock, P A Tresco

  • 1University of Utah, Department of Bioengineering, Salt Lake City 84112, USA.

Journal of Biomaterials Science. Polymer Edition
|August 1, 1998
PubMed
Summary
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Researchers found that Tecoflex SG-80A, an elastomeric polyurethane, supports skeletal myogenesis. This biomaterial is suitable for developing three-dimensional (3-D) cell scaffolds for skeletal muscle tissue engineering.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Understanding skeletal muscle and biomaterial interactions is crucial for developing advanced technologies like cell delivery vehicles and functional muscle tissue.
  • Elastomeric materials are being explored as potential substrates for these applications.

Purpose of the Study:

  • To evaluate the utility of Tecoflex SG-80A, a polyurethane, as a substrate for skeletal myogenesis.
  • To determine if skeletal muscle cells can differentiate and form functional tissue on both two-dimensional (2-D) and three-dimensional (3-D) Tecoflex substrates.

Main Methods:

  • Skeletal myoblasts (G8) were cultured on 2-D Tecoflex thin films fabricated via spin-casting.
  • Porous 3-D cell scaffolds were created using an immersion precipitation method.

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  • Mechanical properties of the 3-D scaffolds were analyzed.
  • Muscle precursors were seeded onto 3-D scaffolds and cultured.
  • Histological and immunohistochemical analyses were performed to assess cell differentiation.

    • Main Results:

    • On 2-D films, myoblasts attached, proliferated, migrated, and differentiated into multinucleated myotubes expressing myosin heavy chain.
    • The 3-D scaffolds exhibited elastomeric properties, recovering original length after 100% elongation.
    • Multinucleated myotubes expressing myosin heavy chain were observed within the 3-D scaffolds after several weeks of culture.

    Conclusions:

    • Tecoflex SG-80A supports skeletal myogenesis, indicating its suitability as a biomaterial for skeletal muscle applications.
    • The fabrication of elastomeric 3-D scaffolds from Tecoflex is feasible.
    • These findings suggest the potential for engineering bioartificial systems using skeletal muscle cultivated on 3-D elastomeric substrates.