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Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
Cytotoxicity of root perforation repair materials
H A Makkawy1, S Koka, M T Lavin
1Department of Surgical Specialties, University of Nebraska Medical Center College of Dentistry, Lincoln 68583-0750, USA.
Journal of Endodontics
|August 7, 1998
Summary
Dental restorative materials, including resin-modified glass ionomer cements and dental amalgam, were tested for cytotoxicity on human periodontal ligament cells. Amalgam showed higher initial inhibition, suggesting R-M GICs may offer a better early biological response for root perforation repair.
Area of Science:
- Dental Materials Science
- Biocompatibility Testing
- Cell Biology
Background:
- Investigating the cytotoxicity of dental restorative materials is crucial for ensuring patient safety and biological compatibility.
- Root perforation repair materials require thorough evaluation for their effects on surrounding cells.
Purpose of the Study:
- To evaluate the in vitro cytotoxicity of two resin-modified glass ionomer cements (R-M GICs) and one dental amalgam on human periodontal ligament (PDL) cells.
- To compare the biological response of PDL cells to these materials when used for root perforation repair.
Main Methods:
- Human PDL cells were exposed to eluates from Fuji Duet, Fuji II LC (R-M GICs), and Contour (dental amalgam) over 24, 48, and 72 hours.
- Cell viability was assessed using the MTS assay, measuring mitochondrial activity.
- Statistical analysis included ANOVA and Tukey's HSD to determine significant differences between material and time effects.
Main Results:
- Both material type and exposure time significantly affected PDL cell viability (p < 0.0001).
- Dental amalgam eluate demonstrated significant inhibition of cell viability at 24 hours compared to controls and R-M GICs.
- At 48 and 72 hours, all tested materials exhibited a similar, mild inhibitory effect on cell viability.
Conclusions:
- Resin-modified glass ionomer cements may elicit a more favorable initial biological response from PDL cells compared to the tested dental amalgam for root perforation repair (< 24 hours).
- Further research into the long-term effects and specific mechanisms of cytotoxicity is warranted.
