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The EGF\EGF-receptor axis modulates enterocyte apoptosis during intestinal adaptation
M A Helmrath1, C E Shin, C R Erwin
1Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45229-3039, USA.
The Journal of Surgical Research
|August 12, 1998
Summary
Epidermal growth factor (EGF) reduces apoptosis after small bowel resection (SBR). Defective EGF receptors worsen apoptosis, highlighting EGF
Area of Science:
- Gastroenterology
- Cell Biology
- Regenerative Medicine
Background:
- Small bowel resection (SBR) triggers adaptive changes in enterocyte proliferation and apoptosis.
- Epidermal growth factor (EGF) is known to promote intestinal adaptation by enhancing proliferation.
Purpose of the Study:
- To investigate the role of EGF receptor signaling in regulating apoptosis following SBR.
- To determine if EGF administration affects apoptosis after SBR.
Main Methods:
- Male ICR mice underwent 50% SBR or sham operation.
- EGF or saline was administered via orogastric gavage.
- Proliferation and apoptosis indices were measured in the ileum; apoptosis was also assessed in mice with defective EGF receptors (waved-2).
Main Results:
- SBR increased enterocyte apoptosis in crypts (cAI) and villi (vAI).
- EGF administration attenuated both cAI and vAI following SBR.
- Mice with defective EGF receptors exhibited significantly higher cAI and vAI after SBR.
Conclusions:
- EGF administration attenuates enterocyte apoptosis during intestinal adaptation after SBR.
- Defective EGF receptors exacerbate apoptosis following SBR.
- EGF's beneficial effect during intestinal adaptation may involve suppression of apoptosis, in addition to enhanced proliferation.