Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lactate production in pancreatic islets

J Tamarit-Rodriguez1, L A Idahl, E Giné

  • 1Department of Biochemistry, School of Medicine, Complutense University, Madrid, Spain. tamarit@eucmax.sim.ucm.es

Diabetes
|August 14, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lymphomas: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Gut microbiota and voluntary alcohol consumption.

Translational psychiatry·2022
Same author

Alcohol mixed with energy drinks: what about taurine?

Psychopharmacology·2020
Same author

Adult-onset hypothyroidism increases ethanol consumption.

Psychopharmacology·2018
Same author

Posttransplant monomorphic Burkitt's lymphoma: clinical characteristics and outcome of a multicenter series.

Annals of hematology·2018
Same author

Clinicobiological features and prognostic impact of diffuse large B-cell lymphoma component in the outcome of patients with previously untreated follicular lymphoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2017

Islets produce lactate even without glucose, with production peaking at low glucose levels. This lactate production influences glucose metabolism and insulin release in both rat and obese mouse models.

Area of Science:

  • Metabolic research
  • Islet cell function
  • Biochemistry

Background:

  • Glucose metabolism in pancreatic islets is crucial for insulin secretion.
  • Understanding glucose handling in islets from different models, like ob/ob mice, is important for diabetes research.

Purpose of the Study:

  • To investigate lactate production and glucose utilization in pancreatic islets from rat and ob/ob mice.
  • To determine the impact of varying glucose concentrations on these metabolic pathways.
  • To examine the role of glucokinase in glucose metabolism using D-mannoheptulose.

Main Methods:

  • Lactate determination using esterification, separation, and high-performance liquid chromatography.
  • Measurement of glucose utilization and oxidation rates.

Related Experiment Videos

  • Assessment of D-mannoheptulose's inhibitory effects on glucose metabolism.

    • Main Results:

    • Significant lactate production occurred in the absence of glucose, saturating at low concentrations (0.2-1.0 mmol/l).
    • Glucose utilization showed a broader concentration dependence (K0.5 between 3-10 mmol/l).
    • D-mannoheptulose inhibited glucose metabolism more significantly at high glucose concentrations, indicating glucokinase's role.

    Conclusions:

    • Islet lactate production saturates at low glucose, potentially favoring oxidative metabolism at higher concentrations.
    • Glucokinase plays a significant role in glucose phosphorylation, particularly at higher glucose levels.
    • These findings provide insights into islet metabolic regulation in normal and obese states.