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Assessment of Kidney Function in Mouse Models of Glomerular Disease
Published on: June 30, 2018
[Activity of endogenous proteinases in glomerulonephritis in children]
O V Chumakova1, A V Kuznetsova, G N Rudenskaia
1Department of Chemistry, Moscow State University, Moscow.
Voprosy Meditsinskoi Khimii
|August 15, 1998
Insights
Serine proteinase activity in children's urine and serum correlates with primary glomerulonephritis and hypertension. This finding links protease levels to specific clinical and morphological disease forms.
Area of Science:
- Biochemistry
- Nephrology
- Pediatrics
Context:
- Glomerulonephritis is a significant cause of kidney disease in children.
- Assessing biomarkers for glomerulonephritis is crucial for diagnosis and management.
- Serine proteinases are implicated in various physiological and pathological processes.
Purpose:
- To investigate the relationship between protease activity in biological samples and the clinical/morphological characteristics of primary glomerulonephritis in children.
- To explore potential diagnostic or prognostic markers for glomerulonephritis.
Summary:
- Protease activities were measured in the serum and urine of 116 children diagnosed with glomerulonephritis and 16 healthy controls.
- Chromogenic peptide substrates (Z-Dala-Leu-Arg-pNA and Glp-Ala-ALa-Leu-pNa) were utilized for activity assays.
- A significant correlation was identified between the activity of serine proteinases and the clinical and morphological presentations of primary glomerulonephritis, including hypertension.
Impact:
- This study highlights the potential of serine proteinases as biomarkers in pediatric glomerulonephritis.
- Findings may contribute to a better understanding of disease pathogenesis and inform clinical stratification.
- Further research could explore therapeutic targeting of these proteases.
Abstract:
Protease activities in serum and urine of 116 children with glomerulonephritis and 16 healthy children were tested using chromogenic peptide substrates Z-Dala_Leu-Arg-pNA and Glp-Ala-ALa-Leu-pNa. We found the dependence between activity of serine proteinases and clinical, morphological forms of primary glomerulonephritis and hypertension.
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