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A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic
A Eggen1, S Solinas-Toldo, R Fries
1Swiss Federal Institute of Technology, ETH Zentrum, Zurich, Switzerland.
The Journal of Heredity
|August 15, 1998
Abstract:
A clone for the bovine gene MAP1B was isolated from a bovine cosmid library with a probe obtained by cross-species PCR. The single positive cosmid clone was localized by FISH to chromosome region 20q14. Analysis of the cosmid revealed the presence of a microsatellite motif with a two-allele polymorphism detectable by PCR amplification of genomic DNA. However, the analysis of this polymorphism in a bovine family affected by SMA indicated absence of very close linkage of the phenotype for bovine SMA with MAP1B.